Connect with us

News

Pooling Five COVID-19 Samples May Not Lead To Much Loss Of Sensitivity Even For Low Viral Loads: ICMR Scientist

The Indian Council of Medical Research (ICMR) conducted a study to understand comparative analysis of pooled testing for five and 10 sample pools by RT-PCR at ten different laboratories conducting COVID-19 testing across the country

Pooling Five COVID-19 Samples May Not Lead To Much Loss Of Sensitivity Even For Low Viral Loads: ICMR Scientist
Highlights
  • The study has been published in the Indian Journal of Medical Research
  • Pooling 5 samples to detect coronavirus may be an acceptable strategy: ICMR
  • Pooling 10 samples could give considerably higher numbers of false negative

New Delhi: A study done by the Indian Council of Medical Research (ICMR) has revealed that pooling five samples to detect coronavirus by RT-PCR may be an acceptable strategy without much loss of sensitivity even for low viral loads, while with 10-sample pools there could be considerably higher numbers of false negatives. The study was done to understand comparative analysis of pooled testing for five- and 10-sample pools by RT-PCR at ten different laboratories conducting COVID-19 testing across the country.

The findings of the study have now been published in the latest edition of the Indian Journal of Medical Research (IJMR).

Also Read: Coronavirus Outbreak Explained: What Is Pool Testing And Can It Expedite COVID-19 Detection In The Country?

Dr Nivedita Gupta, a scientist at ICMR and author of the study, said that the laboratories are facing huge sample loads for COVID-19 diagnosis by RT-PCR.

The high sensitivity of optimized real-time RT-PCR assays makes pooled testing a potentially efficient strategy for resource utilization when positivity rates for particular regions or groups of individuals are low. We conducted a comparative analysis of pooled testing for 5- and 10-sample pools by real-time RT-PCR across 10 COVID-19 testing laboratories in India, she said.

At least ten virus research and diagnostic laboratories (VRDLs) testing COVID-19 by RT-PCR participated in this evaluation.

At each laboratory, 100 nasopharyngeal swab samples including 10 positive samples were used to create 5- and 10-sample pools with one positive sample in each pool. RNA extraction and real-time RT-PCR for SARS-CoV-2-specific E gene targets were performed for individual positive samples as well as pooled samples, Dr Gupta said.

The scientist said that concordance between individual sample testing and testing in the 5- or 10-sample pools was calculated and the variation across sites and by sample cycle threshold (Ct ) values was analyzed.

Results from this multi-site assessment suggest that pooling five samples for SARS-CoV-2 detection by RT-PCR may be an acceptable strategy without much loss of sensitivity even for low viral loads, while with 10-sample pools, there may be considerably higher numbers of false negatives. However, testing laboratories should perform validations with the specific RNA extraction and RT-PCR kits in use at their centres before initiating pooled testing, she said.

Also Read: ‘On-Demand’ COVID-19 Testing For All Individuals, No Delays In Emergency Procedure Due To Lack Of Testing: Health Ministry

(Except for the headline, this story has not been edited by NDTV staff and is published from a syndicated feed.) 

NDTV – Dettol Banega Swasth India campaign is an extension of the five-year-old Banega Swachh India initiative helmed by Campaign Ambassador Amitabh Bachchan. It aims to spread awareness about critical health issues facing the country. In wake of the current COVID-19 pandemic, the need for WASH (WaterSanitation and Hygiene) is reaffirmed as handwashing is one of the ways to prevent Coronavirus infection and other diseases. The campaign highlights the importance of nutrition and healthcare for women and children to prevent maternal and child mortality, fight malnutrition, stunting, wasting, anaemia and disease prevention through vaccines. Importance of programmes like Public Distribution System (PDS), Mid-day Meal Scheme, POSHAN Abhiyan and the role of Aganwadis and ASHA workers are also covered. Only a Swachh or clean India where toilets are used and open defecation free (ODF) status achieved as part of the Swachh Bharat Abhiyan launched by Prime Minister Narendra Modi in 2014, can eradicate diseases like diahorrea and become a Swasth or healthy India. The campaign will continue to cover issues like air pollutionwaste managementplastic banmanual scavenging and sanitation workers and menstrual hygiene.  

 

Click to comment

Leave a Reply

Your email address will not be published.

Folk Music For A Swasth India

RajasthanDay” src=

Reckitt’s Commitment To A Better Future

Expert Blog

हिंदी में पड़े

Latest Posts