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Indigenous ELISA Kits 92.37 Per Cent Sensitive, 97.9 Per Cent Specific For COVID-19 Testing: Indian Council of Medical Research

According to the Indian Council of Medical Research (ICMR), ELISA kits will be useful in screening healthcare workers, industry workers and other people exposed to the Coronavirus

Indigenous ELISA Kits 92.37 Per Cent Sensitive, 97.9 Per Cent Specific For COVID-19 Testing: Indian Council of Medical Research
Highlights
  • ELISA kit found to be 92.37% sensitive, 97.9% specific: ICMR
  • Positive, negative predictive values were 94.44, 98.14% respectively: ICMR
  • IgM/IgG ELISA kits for COVID-19 are in the stage of development: ICMR

New Delhi: Indigenous IgG-based ELISA kits for COVID-19 testing have been found to be sensitive and specific for the detection of coronavirus infection in human serum samples. Hence, these ELISA kits will be useful in screening healthcare workers, industry workers and other people exposed to the virus, according to the Indian Council of Medical Research (ICMR) in its latest scientific paper. A total of 513 blood samples (131 positives, 382 negatives for SARS-CoV-2) were collected and tested by micro-neutralization test (MNT).

“The virus neutralization is regarded as the gold standard method for a serological assay. The in-house ELISA results correlated strongly with neutralization assay, indicating that the assay was sensitive and specific for the detection of anti-SARS-CoV-2 IgG,” it said.

Also Read: Combating COVID-19: NIV-Pune Develops India’s First Antibody Testing Kit ‘ELISA’

The developed ELISA was found to be 92.37 per cent sensitive, 97.9 per cent specific, robust and reproducible. The positive and negative predictive values were 94.44 and 98.14 per cent, respectively, stated ICMR findings.

The assay performance was not different with the use of serum or plasma. No cross-reactivity with other respiratory viruses was detected. This kit may be used to detect exposed immune-protected individuals. This ELISA will be useful in screening healthcare workers, industry workers, etc.

According to the apex medical research body, for surveillance of the emerging and re-emerging viruses, serological assays have been widely recommended. Several IgM/IgG ELISA kits for COVID-19 are in the stage of development.

Also Read: India Testing 1.2 Lakh COVID-19 Samples Daily, Far From Peak In Novel Coronavirus Cases: ICMR

However, validated and approved SARS-CoV-2 serological assays are lacking for case detection and are not included in laboratory testing guidelines for COVID-19 of the WHO.

“Due to the non-availability of an indigenous, approved and cost-effective kit, an in-house ELISA was developed and validated for the detection of anti-SARS-CoV-2 human IgG antibodies,” read the paper. The ELISA kits can be produced at lower manufacturing cost. It gives easy-to-use and affordable kits to resource-limited settings.

The study concluded that this indigenous anti-SARS-CoV-2 human IgG-ELISA may be used for ascertaining the seroprevalence against SARS-CoV-2 in a population and for epidemiological studies.

Also Read: ICMR Advises States To Conduct Sero-Survey To Measure Coronavirus Exposure In People Using IgG ELISA Test

World

26,14,68,997Cases
22,22,61,229Active
3,40,08,183Recovered
51,99,585Deaths
Coronavirus has spread to 196 countries. The total confirmed cases worldwide are 26,14,68,997 and 51,99,585 have died; 22,22,61,229 are active cases and 3,40,08,183 have recovered as on November 29, 2021 at 3:42 am.

India

3,45,72,523 8,774Cases
1,05,6911,328Active
3,39,98,278 9,481Recovered
4,68,554 621Deaths
In India, there are 3,45,72,523 confirmed cases including 4,68,554 deaths. The number of active cases is 1,05,691 and 3,39,98,278 have recovered as on November 28, 2021 at 2:30 am.

State Details

State Cases Active Recovered Deaths
Maharashtra

66,33,612 507

11,905 248

64,80,799 738

1,40,908 17

Kerala

51,29,359 4,741

49,152 957

50,40,528 5,144

39,679 554

Karnataka

29,95,285 322

6,783 143

29,50,306 176

38,196 3

Tamil Nadu

27,24,731 740

8,382 36

26,79,895 765

36,454 11

Andhra Pradesh

20,72,446 248

2,158 5

20,55,856 253

14,432

Uttar Pradesh

17,10,373 5

86 5

16,87,377 9

22,910 1

West Bengal

16,14,152 701

7,820 27

15,86,882 717

19,450 11

Delhi

14,40,834 27

290 11

14,15,448 37

25,096 1

Odisha

10,48,492 264

2,222 6

10,37,864 255

8,406 3

Chhattisgarh

10,06,733 27

326 7

9,92,814 20

13,593

Rajasthan

9,54,741 26

187 13

9,45,599 13

8,955

Gujarat

8,27,382 28

291 17

8,16,999 45

10,092

Madhya Pradesh

7,93,120 23

112 9

7,82,480 14

10,528

Haryana

7,71,654 11

159 1

7,61,441 10

10,054

Bihar

7,26,212 3

39 6

7,16,510 9

9,663

Telangana

6,75,479 160

3,545 11

6,67,946 148

3,988 1

Assam

6,16,435 123

2,720 71

6,07,624 189

6,091 5

Punjab

6,03,190 17

313 22

5,86,284 39

16,593

Jharkhand

3,49,216 20

109 0

3,43,967 20

5,140

Uttarakhand

3,44,183 14

150 6

3,36,626 8

7,407

Jammu And Kashmir

3,36,386 149

1,724 5

3,30,189 141

4,473 3

Himachal Pradesh

2,26,941 82

809 18

2,22,287 97

3,845 3

Goa

1,78,839 40

275 9

1,75,183 31

3,381

Mizoram

1,34,279 358

4,117 110

1,29,672 466

490 2

Puducherry

1,28,860 35

326 5

1,26,662 30

1,872

Manipur

1,25,117 19

673 1

1,22,474 18

1,970 2

Tripura

84,784 13

89 9

83,874 3

821 1

Meghalaya

84,414 20

308 27

82,635 44

1,471 3

Chandigarh

65,443 5

52 4

64,571 1

820

Arunachal Pradesh

55,269 9

37 4

54,952 5

280

Sikkim

32,211 4

114 5

31,694 9

403

Nagaland

32,100 4

135 1

31,269 5

696

Ladakh

21,494 27

249 6

21,032 21

213

Dadra And Nagar Haveli

10,683

1 0

10,678

4

Lakshadweep

10,394

28 1

10,315 1

51

Andaman And Nicobar Islands

7,680 2

5 2

7,546

129

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